Plant Transcription Factor Database
Previous version: v3.0
Citrus sinensis
HSF Family
Species TF ID Description
orange1.1g010166mHSF family protein
orange1.1g011077mHSF family protein
orange1.1g011260mHSF family protein
orange1.1g015162mHSF family protein
orange1.1g015604mHSF family protein
orange1.1g015631mHSF family protein
orange1.1g015635mHSF family protein
orange1.1g015644mHSF family protein
orange1.1g015653mHSF family protein
orange1.1g015655mHSF family protein
orange1.1g015665mHSF family protein
orange1.1g015923mHSF family protein
orange1.1g016091mHSF family protein
orange1.1g016378mHSF family protein
orange1.1g016690mHSF family protein
orange1.1g016692mHSF family protein
orange1.1g016698mHSF family protein
orange1.1g016701mHSF family protein
orange1.1g016719mHSF family protein
orange1.1g016828mHSF family protein
orange1.1g017694mHSF family protein
orange1.1g017721mHSF family protein
orange1.1g017908mHSF family protein
orange1.1g018006mHSF family protein
orange1.1g018107mHSF family protein
orange1.1g018495mHSF family protein
orange1.1g020146mHSF family protein
orange1.1g025781mHSF family protein
orange1.1g026263mHSF family protein
orange1.1g032729mHSF family protein
orange1.1g034429mHSF family protein
orange1.1g046676mHSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645